Review



ha tagged map3ks  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 85
      Buy from Supplier

    Structured Review

    Addgene inc ha tagged map3ks
    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Ha Tagged Map3ks, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv5+ha+mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/bio_rxiv__64898__2026__03__09__710604-214-13-24
    Average 85 stars, based on 2 article reviews
    ha tagged map3ks - by Bioz Stars, 2026-10
    85/100 stars

    Images

    1) Product Images from "Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death"

    Article Title: Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death

    Journal: bioRxiv

    doi: 10.64898/2026.03.09.710604

    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Figure Legend Snippet: A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.

    Techniques Used: Transfection, Western Blot, Activation Assay, Control, Binding Assay, Expressing

    A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.
    Figure Legend Snippet: A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.

    Techniques Used: Sequencing, Derivative Assay, Transfection, Control, Activation Assay, Immunoprecipitation, Binding Assay

    Related Articles

    Plasmid Preparation:

    Article Title: MEKK2 Kinase Association with 14-3-3 Protein Regulates Activation of c-Jun N-terminal Kinase
    Article Snippet: .. pCMV5-HA-MEKK2 was purchased from Addgene (Boston, MA; plasmid 12182). ..



    Similar Products

    ha tagged map3ks  (Addgene inc)
    85
    Addgene inc ha tagged map3ks
    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Ha Tagged Map3ks, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv5+ha+mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/bio_rxiv__64898__2026__03__09__710604-214-13-24
    Average 85 stars, based on 1 article reviews
    ha tagged map3ks - by Bioz Stars, 2026-10
    85/100 stars
    		  Buy from Supplier
    pcmv5 ha mekk2  (Addgene inc)
    85
    Addgene inc pcmv5 ha mekk2
    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Pcmv5 Ha Mekk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv5+ha+mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/pmc03784737-82-0-4
    Average 85 stars, based on 1 article reviews
    pcmv5 ha mekk2 - by Bioz Stars, 2026-10
    85/100 stars
    		  Buy from Supplier
    mutagenesis pcmv5 ha mekk2  (Addgene inc)
    85
    Addgene inc mutagenesis pcmv5 ha mekk2
    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Mutagenesis Pcmv5 Ha Mekk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv5+ha+mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/pmc03784737-39-2-7
    Average 85 stars, based on 1 article reviews
    mutagenesis pcmv5 ha mekk2 - by Bioz Stars, 2026-10
    85/100 stars
    		  Buy from Supplier
    mutagenesis  (Addgene inc)
    85
    Addgene inc mutagenesis
    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated <t>HA-MAP3Ks.</t> Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.
    Mutagenesis, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv5+ha+mekk2/pCMV5+MEKK2+WT+(Plasmid+%2312182)/10__1074_slash_jbc__m113__511352-33-2-6
    Average 85 stars, based on 1 article reviews
    mutagenesis - by Bioz Stars, 2026-10
    85/100 stars
    		  Buy from Supplier

    Image Search Results


    A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.

    Journal: bioRxiv

    Article Title: Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death

    doi: 10.64898/2026.03.09.710604

    Figure Lengend Snippet: A . AKO cells were co-transfected with Venus-arrestin-3 and indicated HA-MAP3Ks. Venus-arrestin-3 was precipitated using GFP-trap beads and bound HA-MAP3Ks were detected by western blotting. B. Ǫuantification of A (N=3). C. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in AKO cells co-transfected with HA-JNK3α2, indicated HA-MAP3Ks, and Venus (Ve) or Venus-arrestin-3 (Ve-arr3). JNK3 activation was determined by immunoblotting for doubly phosphorylated JNK (ppJNK). D, E. Ǫuantification of C. Bar graphs show JNK3 activation normalized to total HA-JNK3α2 ( D ) or the loading control GAPDH ( E ) (means +/− SEM; N=3). F. MAP3K binding vs arrestin-3-dependent increase in JNK3 activation in AKO cells. G. Arrestin-3 effect on JNK3 activation driven by different MAP3Ks in Neuro2a cells. Experiments were performed as in C. H, I. Ǫuantification of G normalized to total HA-JNK3α2 ( H ) or GAPDH ( I ). J. Relative expression of endogenous MAP3Ks in different cell lines (HEK293, Neuro2a, LAN5, SMS-KCN) (quantification strategy is shown in Figs. S3, S4). For panels B, D, E, H , and I , statistical significance of the differences between Ve and Ve-Arr3 within each specific MAP3K transfection group was determined using multiple unpaired t-tests with Welch’s correction, and the False Discovery Rate (FDR) was set at 5%; p values are indicated.

    Article Snippet: Expression constructs encoding N-terminally Venus-tagged arrestin-3 and HA-ASK1 were described earlier ( ); HA-tagged MAP3Ks (ZAKα, ZAKβ, MEKK1, MEKK2, MEKK4, TAK1) were obtained from Addgene (#12182, 12187, 21632, 44160, 141193, 141195, respectively).

    Techniques: Transfection, Western Blot, Activation Assay, Control, Binding Assay, Expressing

    A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.

    Journal: bioRxiv

    Article Title: Arrestin-3 scaffolds multiple MAP3Ks driving stress-induced JNK3 activation and cell death

    doi: 10.64898/2026.03.09.710604

    Figure Lengend Snippet: A . Structure of arrestin-3 (PDB ID 3P2D) with the sequence corresponding to the N-terminal T1A peptide shown in blue. B. Amino acid sequences of arrestin-3 (Arr3) and derived peptides. C. Neuro2a cells were transfected with HA-JNK3α2, indicated MAP3Ks, and Venus-tagged peptides or Venus (control). Violin plots show the distribution of JNK3α2 activation effects (shown as % of full-length arrestin-3 efficacy indicated as vertical dotted lines). Blots and quantification are in Figs. S6, S7 . D. Co-immunoprecipitation was performed from AKO cells transfected with Venus (Ve) or Venus-T16 (Ve-T16) and HA-ZAKα or HA-ZAKβ. E. Ǫuantification of the binding of Venus-T16 to ZAKα and ZAKβ. Data represent means ± SEM (N= 3). Statistical significance was determined by multiple unpaired t-tests with FDR set at 5%; p-values are indicated above bars.

    Article Snippet: Expression constructs encoding N-terminally Venus-tagged arrestin-3 and HA-ASK1 were described earlier ( ); HA-tagged MAP3Ks (ZAKα, ZAKβ, MEKK1, MEKK2, MEKK4, TAK1) were obtained from Addgene (#12182, 12187, 21632, 44160, 141193, 141195, respectively).

    Techniques: Sequencing, Derivative Assay, Transfection, Control, Activation Assay, Immunoprecipitation, Binding Assay